In a groundbreaking study published in the prestigious scientific journal Analytica Chimica Acta, a team of researchers from the State Key Laboratory of Food Science and Resources at Jiangnan University in China, along with a contributor from the Xinjiang Academy of Agriculture and Reclamation Sciences, unveiled a novel approach to detect acrylamide in food. This new method, described as an enzyme-cascade colorimetric aptasensor, is set to change the game in food safety monitoring.

Acrylamide: A Silent Threat in Our Food

The Maillard reaction, a chemical process involving the reaction of free amino groups with reducing sugars or carbonyl compounds, is a critical step in cooking. It not only provides that desirable golden-brown color and rich flavor to our foods but, as the flip side of the coin, can create hazardous compounds. Acrylamide (AAm) is one such by-product with significant health implications, including neurotoxicity and potential carcinogenicity, linked to mutations and altered gene expression that may expedite organ aging in humans.

Increasing public concern and stricter regulatory standards have put pressure on the food industry and scientists to develop accurate, reliable, and sensitive methods to detect acrylamide levels and ensure consumer safety.

The Innovation: MGzyme Cascade Colorimetric Aptasensor

The new detection method introduced by the team consists of a colorimetric aptasensor, which incorporates a unique combination of MIL-glucose oxidase (MGzyme) hybridized with complementary DNA (cDNA) and a magnetic nanoparticle-aptamer (MNP-Apt) [5]. This innovative system specifically targets acrylamide molecules, binding with them and releasing the MGzyme-cDNA into the mixture.

Upon addition of glucose and tetramethylbenzidine (TMB), the MGzyme gets to work, oxidizing the glucose and generating hydroxyl radicals which, in turn, cause the colorless TMB to convert to blue oxidized TMB. The absorbance of this colored product at 652 nm is directly proportional to the concentration of acrylamide present in the sample, providing a visible indication of its level.

Remarkably, this technology eliminates the need for hydrogen peroxide (H2O2), a potentially harmful intermediate commonly used in other similar assays. Instead, the MGzyme-cDNA system avoids these dangerous substances, improving overall reaction efficacy and the safety of the detection process itself.

A Beacon in Food Safety

The potential applications of this new colorimetric aptasensor are vast. With its remarkable sensitivity and specificity, it represents a significant step forward in enzymatic-cascade systems and their application in detecting a variety of substrates. Most importantly, it addresses the critical need for safe, accurate detection of harmful substances such as acrylamide in the food industry.

The simplicity and effectiveness of this system could see wide-scale adoption in food safety inspection workflows, ultimately leading to better protection for consumers against the risks posed by acrylamide consumption. Moreover, the implications of this development are not limited to food science. The research paves the way for future innovations in biosensors and analytical chemistry, where similar hybrids of aptamers and enzyme-cascade systems could be tailored to detect a wide array of substances with high precision.


1. Acrylamide detection in food
2. Colorimetric aptasensor for food safety
3. Enzyme-cascade biosensors
4. Maillard reaction hazards
5. Magnetic nanoparticle-aptamer in biosensing

Expanding into the Future

Such an innovation is a beacon in the continuous effort to safeguard public health regarding food consumption. As the industry progresses, this aptasensor technology could be integrated into automated systems for real-time monitoring, providing instant feedback on acrylamide levels during food processing.

In conclusion, the research carried out by the Jiangnan University team marks a significant milestone in analytical chemistry and food safety. It reflects a well-coordinated effort where science meets societal needs, edging closer to the ultimate goal of a world where the food we enjoy not only tantalizes our taste buds but is also free from hidden chemical threats.


1. Ma, K., Lin, X., Duan, N., Lu, C., Wang, Z. & Wu, S. (2024). Detection of acrylamide in food based on MIL-glucose oxidase cascade colorimetric aptasensor. Analytica Chimica Acta, 1288, 342150.
2. Mottram, D. S. (1994). Flavour formation in meat and meat products: a review. Food Chemistry, 51(2), 207-214. DOI:
3. Rice, J. M. (2005). The carcinogenicity of acrylamide. Mutation Research/Fundamental and Molecular Mechanisms of Mutagenesis, 580(1-2), 3-20. DOI:
4. European Food Safety Authority. (2015). Scientific Opinion on acrylamide in food. EFSA Journal, 13(6), 4104. DOI:
5. Zhang, Y., et al. (2017). DNA aptamers in the detection of contaminants. Analytica Chimica Acta, 963, 1-17. DOI: